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MAP-5 10ml Vial is a patented salt of hyaluronic acid in normal saline for use in the collection, handling, culture and cryopreservation of embryo, ova, sperm and other cells. Sterile MAP-5 acts as a replacement for serum and serum products in cell handling and freezing solution, and thereby reduces the risk of contaminating microorganisms that may contaminate serum product.
MAP-5 Sterile solution indecated for embryo transfer, in vitro, cellular handling and cryopreservation. Hyaluronic acid is a natural, highly charged, polyanionic molecule composed of alternating units of D-glucuronic and 2-acetamido-2-deoxy-D-glucose. These unbranched, coiled, elongated polysaccharide chains maintain a large negative electrostatic charge that attracts water molecules and allow the deformation of the molecular coil as ice crystallization occurs during freezing and thawing.
Hyaluronic acid coats and protects cells and tissues by attaching to the CD44 (hyaluronan) receptor sites on cells. The purity, defined molecular weight, and molecular chain length of the sodium hyaluronic acid in MAP®-5 ensure that cells are completely coated and protected during all phases of handling and storage.
MAP-5 should be added to collection, handling, culture and freezing solutions to replace serum and serum products normally used in these media. MAP-5 is compatible with all common cell supporting media, cell surfactants and cryopreservative solutions used in embryo transfer and in vitro fertilization procedures. The inclusion concentration depends on the procedure and the prior results and experiences of individual veterinarians and cell biologists. Studies have demonstrated that a 0.1% w/v MAP®-5 replaces 20% v/v of serum in holding and cryopreservative solutions. The common final concentration of MAP®-5 used in freezing solutions is 1 mg/mL (0.1% w/v). Flushing and collection solutions commonly employ 0.1 to 0.5 mg/mL of MAP-5.
Use strict aseptic techniques to add the required amount of MAP-5 to the appropriate handling or freezing solution.
Precaution(s): Store at 60-85°F (15-30°C). MAP-5 contains no preservatives. Discard unused portions of the vials.
Warning(s): For use in embryo transfer procedures, in vitro cellular handling and the cryopreservation of embryo, ova, sperm and other cells and tissues. Not for use as a therapeutic in animals or humans.
MAP-5/50 (5 mg/mL) 10 mL vial - one vial/100-500 mL flushing solution. |
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