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Snap Fiv/Felv Test Test Kits
SNAP FIV/FeLV Test Item# 28817M
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The SNAP Combo FIV Ab/FeLV Ag Test is a rapid immunoassay for simultaneous detection of antibody to feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) antigen in feline serum, plasma or whole blood.
This test detects FeLV p27 antigen and specific antibodies to FIV. The presence of FeLV p27 antigen is diagnostic for FeLV infection and the presence of specific antibodies to FIV indicates that the cat has been exposed to FIV and may have an active FIV infection.

SNAP devices and test reagents are stable until the expiration date when stored at 2°–7°C (35°–45°F).
  • The SNAP ELISA platform provides accurate, in-house results in 10 minutes.
  • One test, using one blood sample, delivers two results for maximum efficiency in your workflow.
  • Effective care and management can help infected cats live long, healthy lives.
  • Clients are willing to pay more for immediate, more thorough screening, with answers during the patient visit.
  • Use the time while the test is running to consult with pet owners on other medical needs, providing a more valuable patient visit.


Idexx Labs.

Feline Leukemia Virus Antigen/Feline Immunodeficiency Virus Antibody Test Kit

For veterinary use only.

SNAP* Combo

The SNAP Combo FeLV Ag/FIV Antibody Test is a rapid immunoassay for simultaneous detection of feline leukemia virus (FeLV) antigen and antibody to feline immunodeficiency virus (FIV) in feline serum, plasma or whole blood. The presence of FeLV p27 antigen is diagnostic for FeLV infection, and the presence of specific antibodies to FIV indicates that a cat has been exposed to FIV and may have an active FIV infection.

Kit Components

Each kit contains 5, 15 or 30 SNAP devices, as well as one 3.5-mL or 7-mL bottle of anti-FeLV/FIV Ag:HRPO conjugate, a reagent rack, transfer pipettes and sample tubes. Each SNAP device contains 0.4 mL of wash solution and 0.6 mL of substrate solution.


Store devices and test reagents at 2°-7°C (35°-45°F). All components must be at room temperature (15°-30°C/59°-86°F) before running the test-do not heat.

Precautions and Warnings

- FIV antigen used in the conjugate has been chemically inactivated.

- Do not mix components from kits with different lot numbers.

- Do not use a SNAP device that has been activated prior to the addition of sample.

Sample Information

- Samples must be at room temperature (15°-30°C/59°-86°F) before beginning the test procedure.

- Serum, plasma or anticoagulated whole blood (e.g., EDTA, heparin), either fresh or stored at 2°-7°C (35°-45°F) for up to one week, can be used.

- For longer storage, serum or plasma can be frozen (-20°C/-4°F or colder) and then recentrifuged before use.

- Hemolyzed or lipemic samples will not affect results.

Test Procedure

1. Allow all components to equilibrate at room temperature (15°-30°C/59°-86°F) for 30 minutes before use. Do not heat.

2. Using the pipet that is provided, dispense 3 drops of sample into a new sample tube.


3. Holding the bottle vertical, add 4 drops of conjugate to the sample tube.


4. Cap the sample tube and mix it thoroughly by inverting it 3-5 times.


5. Place the device on a horizontal surface. Add the entire contents of the sample tube to the sample well, being careful not to splash the contents outside of the sample well.

The sample will flow across the result window, reaching the activation circle in 30-60 seconds. Some sample may remain in the sample well.


6. When color FIRST appears in the activation circle, push the activator firmly until it is flush with the device body.


NOTE: Some samples may not flow to the activation circle within 60 seconds, and, therefore, the circle may not turn color. In this case, press the activator after the sample has flowed across the result window.

7. Test results must be read at 10 minutes from the time of activation

NOTE: The positive control may develop sooner, but results are not complete until 10 minutes.

Interpreting Test Results

Positive Result

Any color development in the sample spots indicates the presence of FIV antibody or FeLV antigen in the sample.


Negative Result

Only the positive control spot develops color.


Reaction with Negative Control

The negative control spot serves as a safeguard against diagnosing false positives, and helps indicate that the assay has been run properly.

Invalid Result

If color in the negative control spot is equal to or darker than the FIV antibody or FeLV antigen sample spot, the test is invalid for that spot.


Invalid Results

1. Background-If the sample is allowed to flow past the activate circle, background color may result. Some background color is normal. However, if colored background obscures test result, repeat the test.

2. No Color Development-If the positive control does not develop color, repeat the test.

Sensitivity and Specificity

Comparison Test

Sample Size
SNAP Combo Test/Reference Test

Sample Type

Relative Sensitivity and Specificity
95% Confidence Limit

Kappa Statistic






PetChek FeLV ag






Serum/Plasma/Whole Blood

Sen., 97.6% (95% CL 91.1%-99.8%)

Spec., 99.1% (95% CL 96.4%-99.9%)


PetChek FIV






Serum/Plasma/Whole Blood

Sen., 100% (95% CL 95.3%-100.7%)

Sen., 99.5% (95% CL 96.9%-100.2%)


CL = confidence limit

IDEXX Customer Support

U.S./Canada 1-800-248-2483

Europe 00-800-1234-3399

Australia 1800-655-978

U.S. Vet. License No. 313

Product Code 502A.02

*SNAP and PetChek are trademarks or registered trademarks of IDEXX Laboratories, Inc. in the United States and/or other countries.

Covered by U.S. Patent Nos. 4,713,325; 5,037,753; 5,118,602; 5,627,026; 5,726,010; 5,726,013; 5,750,333 and/or 6,007,999. Other U.S. and/or foreign patents issued or pending.

© 2006 IDEXX Laboratories, Inc. All rights reserved.


IDEXX LABORATORIES, One IDEXX Drive, Westbrook, Maine 04092 USA

NAC No.: 11160483

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