NEVER SHARE NEEDLES AND SYRINGES.
Correct Syringe Type
Doses of insulin are measured in units. U-100 insulin contains 100 units/mL (1 mL=1 cc).
With Humulin N, it is important to use a syringe that is marked for U-100 insulin preparations.
Failure to use the proper syringe can lead to a mistake in dosage, causing serious problems for
your pet, such as a blood glucose level that is too low or too high.
To help avoid contamination and possible infection, follow these instructions exactly.
Disposable syringes and needles should be used only once and then discarded by placing the
used needle in a puncture-resistant disposable container. Properly dispose of the punctureresistant container as directed by your Health Care Professional.
Preparing the Dose
1. Wash your hands.
2. Carefully rotate the bottle of insulin several times to completely mix the insulin.
3. Inspect the insulin. Humulin N suspension should look uniformly cloudy or milky. Do not
use Humulin N if you notice anything unusual in its appearance.
4. If using a new Humulin N bottle, flip off the plastic protective cap, but do not remove the
stopper. Wipe the top of the bottle with an alcohol swab.
5. If you are mixing insulins, refer to the “Mixing Humulin N and Regular Human Insulin”
section below. 3
6. Draw an amount of air into the syringe that is equal to the Humulin N dose. Put the needle
through rubber top of the Humulin N bottle and inject the air into the bottle.
7. Turn the Humulin N bottle and syringe upside down. Hold the bottle and syringe firmly in
one hand and shake gently.
8. Making sure the tip of the needle is in the Humulin N suspension, withdraw the correct
dose of Humulin N into the syringe.
9. Before removing the needle from the Humulin N bottle, check the syringe for air bubbles.
If bubbles are present, hold the syringe straight up and tap its side until the bubbles float
to the top. Push the bubbles out with the plunger and then withdraw the correct dose.
10. Remove the needle from the bottle and lay the syringe down so that the needle does not
11. If you do not need to mix your Humulin N with Regular human insulin, go to the
“Injection Instructions” section below and follow the directions.
Do not use Humulin N:
• if the insulin substance (the white material) remains at the bottom of the bottle after mixing
• if there are clumps in the insulin after mixing, or
• if solid white particles stick to the bottom or wall of the bottle, giving a frosted appearance.
If you see anything unusual in the appearance of Humulin N suspension in your bottle or notice
your insulin requirements changing, talk to your veterinarian