
SPECIMEN COLLECTION
1. Fresh samples should be used when possible. If samples cannot be used within 24 hours, they should be stored cold (2°-8°C or 36°-46°F) for up to two weeks, or kept frozen before being tested.
2. Some fecal samples contain mucous. In order to collect an accurate sample, avoid coating the swab completely with mucous material.
TEST PROCEDURE
1. Remove the sampling swab from the dilution chamber of the Sample Dilution Device. Coat the sampling swab with a thin layer of fecal material or saturate the swab if the sample is liquid. Return the sampling swab back into the dilution chamber.
2. Break the seal of the Extraction Buffer storage bulb located on the top of the Sample Dilution Device by bending the bulb back and forth until the internal blue stick breaks at its base. Squeeze the bulb 5 - 6 times to thoroughly mix the sample with the Extraction Buffer.
3. Remove a Test Device from the protective pouch and place it on a flat surface.
4. Using the buffer storage bulb and the sampling swab as a pipette, transfer 3 drops (~150µL) of the diluted sample into the round sample well of the Test Device. Hold the pipette vertically to ensure proper sample volume. Start timing.
5. Read the results between 10 minutes and 15 minutes. Strong positive results may appear as soon as 1 minute, and weak positive results may take up to 10 minutes to appear. After 15 minutes, discard the Test Device because the results are no longer valid.
INTERPRETATION OF RESULTS
The result is negative when one red to pink line appears in the C (control) area.
The result is positive when two red to pink lines appear. One red/pink line will appear in the T (test) area and one in the C (control) area. A red to pink line with any intensity in the T (test) area should be considered positive. Colored lines may be lighter or darker than each other.
The test is invalid when no colored line appears in the C (control) area, even if a red to pink line appears in the T (test) area. Repeat the test using a new cassette. Colored lines that appear after 15 minutes are not diagnostic and should be ignored.
VetScan Canine Rapid Parvovirus Test Kit, 10 Tests